E2F1 anticorps (AA 223-332)

N° du produit ABIN7465667

Cet anticorps anti-E2F1 est un anticorps Souris Monoclonal détectant E2F1 dans WB, IP, IF et ICC. Adapté pour Humain.

Aperçu rapide pour E2F1 anticorps (AA 223-332) (ABIN7465667)

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp E2F1 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 10D1 2B5

Détail du produit anti-E2F1 anticorps

Épitope: AA 223-332

Réactivité croisée: Humain

Purification: Protein G purified

Immunogène: The region encoding the DP-1 binding site (amino acids 223-332) of E2F-1 expressed in E. coli.

Isotype: IgG2b

Information d'application

Indications d'application: WB: 1 - 5 μg/mL. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: E2F-1 transfected Soas2 cells

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, No Preservative

Agent conservateur: Without preservative

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur E2F1

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Autre désignation: E2F transcription factor 1

Sujet: E2F transcription factor 1 , E2F-1 , RBAP1 , RBBP3 , RBP3,The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Poids moléculaire: 47 kDa

ID gène: 1869

UniProt: Q01094

Pathways: Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy